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d <- read.table('len_width.txt', header = TRUE) d.sp1 <- d[d$sp == 'Sp1', ] d.sp2 <- d[d$sp == 'Sp2', ] plot(d.sp1$len, d.sp1$width, pch = 16, col = 'black', xlim = c(0, 80), ylim = c(0, 30), xlab = 'Length', ylab = 'Width') par(new = T) plot(d.sp2$len, d.sp2$width, pch = 16, col = 'red', xlim = c(0, 80), ylim = c(0, 30), xlab = '', ylab = '')